Brain Organoids and 3D Neuronal Cultures

Generate electrophysiology maps for precise brain imaging in vitro. Record electrophysiological activity in brain spheroids, brain organoids and retinal organoids.

Models

Brain/CorticalOrganoids
Retinal Organoids
Spheroids
Scaffold/matrix-based 3D cultures

Advantages

In vitro Brain Organoids or Spheroids reproduce key brain in-vivo organization features as cell–cell 3-dimensional interactions or tissue-specific architecture. This makes models a great resource for assessing the efficacy of drugs in brain disease studies and for toxicological and compound screening. 3Brain’s single-well and multiwell CorePlate™ can measure functional activity from brain organoids at an unprecedented high spatial resolution.

Advantages of 3Brain technology with brain organoids are:

Label-free functional monitoring of whole organoids with single-cell spiking activity resolution.
Improved statistical significance measuring from thousands of neurons simultaneously
Multiple sample recordings: 3Brain’s single-well CorePlate™ has a sensing area of up to 25mm², allowing to record multiple spheroids in a single experiment.

Click here to know how the technology works

Tools & Features

Integrated spike and field detection algorithms within the BrainWave software - 3Brain

Integrated spike and field detection algorithms
BrainWave software detects, extracts and classifies spikes or field potentials from organoids.

Superimpose and Focus on BrainWave - 3Brain

Superimpose and Focus
BrainWave integrates advanced tools to superimpose images of your samples on the activity map and zoom on it. This feature allows you to record the selected region of interestor to focus on a specific area of activity for advanced analysis.

Automated statistical analysis of network parameters - 3Brain

Automated statistical analysis of network parameters
The all-in-one BrainWave softwarehelps the user to quickly obtain results from an experimental session in asimple and intuitive way.

Ability to design your experimental session through the BrainWave software - 3Brain

Design your experimental session
The BrainWave intuitive graphical user interface gives the user full control of the experimental designto rapidly obtain results from an experimental session.

Feel free to get in contact with our neuroscientists team for details

Customer experience

A collection of reviews from our customers

The Muotri lab uses 3Brain high-resolution multi electrode arrays to understand how human neural networks are formed during development, and become altered in several neurological conditions.

Prof. Alysson Muotri

Institute for Genomic Medicine Link to Author’s Publication

It is a pleasure to work with the 3Brain team, they are both highly skilled experts and friendly.

Dr. Fred Jordan, Co-CEO

FinalSpark

The BioCAM offers, for the first time, recordings at a scale that allow validation of models of neuronal population activity. This is a major step that will drive, in turn, the development of theories in neuroscience.

Dr. Matthias H. Hennig

Computational Neuroscience and Neuroinformatics Link to Author’s Publication

Our laboratory works on brain experimental recordings and modeling on multiple scales, from molecular/cellular to whole brain in animals and humans. The use of BioCAM X integrates perfectly into the workflow as it allows a unique set of measurements on distributed network activities.

Prof. Egidio D’Angelo

University of Pavia Link to Author’s Publication

The BioCAM has revolutionized the way we look at population activity in the retina. With its overall large size and spatial resolution, it allows us to study population behaviour at unprecedented spatial and temporal detail.

Prof. Evelyne Sernagor

Retinal Neuroscience Link to Author’s Publication

We engineer a novel plasticity neuroelectronic platform using CMOS-MEA, nano-microtechnology and computational tools to decipher the molecular and functional plasticity simultaneously in a large-scale circuit/network in health and disease.

Dr. Hayder Amin

Biohybrid Neuroelectronics Lab Link to Author’s Publication

I have conducted a wide range of brain slices as well as neuron culture experiments in 3Brain's HD MEA system. Their technology is beyond expectation in terms of sensitivity, durability, and high STN ratio.

Prof. Kim SangSeong

Hanyang University College of Pharmacy

3Brain has impressed me with the quality of its products. The BioCAM DupleX has been the perfect platform to aid our drug discovery efforts at Xenon, and the personal one-on-one assistance continues to exceed my expectations.

Dr. Ryley Parrish

Xenon Pharmaceuticals Inc.

The HD MEAs allow us to perform accurate cardiotoxicity assessments on hiPS cardiomyocytes by applying a novel laser technology for recording high-quality action potentials from thousands of cardiac cells simultaneously.

Michele Dipalo, PhD

Plasmon Nanotechnologies group at the Italian Institute of Technology (IIT)Link to Author’s Publication

Resources

Location to specific products and samples within 3Brain's resources

Selected Publications

View all

Organoids

Generation of human region-specific brain organoids with medullary spinal trigeminal nuclei

(paper)

Wei Pang, Jinkui Zhu, Kexin Yang, Xiaona Zhu, Wei Zhou, Linlin Jiang, Xuran Zhuang, Yantong Liu, Jianfeng Wei, Xiaoxiang Lu, Yao Yin, Ziling Chen, Yangfei Xiang

Cell Stem Cell (2024). DOI: 10.1016/j.stem.2024.08.004

Organoids

Brain Chimeroids reveal individual susceptibility to neurotoxic triggers

(paper)

Noelia Antón-Bolaños, Irene Faravelli, Tyler Faits, Sophia Andreadis, Rahel Kastli, Sebastiano Trattaro, Xian Adiconis, Anqi Wei, Abhishek Sampath Kumar, Daniela J. Di Bella, Matthew Tegtmeyer, Ralda Nehme, Joshua Z. Levin, Aviv Regev & Paola Arlotta

Nature (2024). DOI: 10.1038/s41586-024-07578-8

Organoids

Ataxia Telangiectasia patient-derived neuronal and brain organoid models reveal mitochondrial dysfunction and oxidative stress

(paper)

Hannah C. Leeson, Julio Aguado, Cecilia Gómez-Inclán, Harman Kaur Chaggar, Atefah Taherian Fard, Zoe Hunter, Martin F. Lavin, Alan Mackay-Sim, Ernst J. Wolvetang

Neurobiology of Disease Vol. 199 (2024). DOI: https://doi.org/10.1016/j.nbd.2024.106562

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Probing intelligent networks

Brain Organoids and 3D Neuronal Cultures

Models

Advantages

Tools & Features

Customer experience

Resources

Selected Publications

Organoids

Generation of human region-specific brain organoids with medullary spinal trigeminal nuclei

Generation of human region-specific brain organoids with medullary spinal trigeminal nuclei

Organoids

Brain Chimeroids reveal individual susceptibility to neurotoxic triggers

Brain Chimeroids reveal individual susceptibility to neurotoxic triggers

Organoids

Ataxia Telangiectasia patient-derived neuronal and brain organoid models reveal mitochondrial dysfunction and oxidative stress

Ataxia Telangiectasia patient-derived neuronal and brain organoid models reveal mitochondrial dysfunction and oxidative stress

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